Forschungsprojekt: RESET: ESBL and (fluoro)quinolone resistance in Enterobacteriaceae
Resistances to ß-lactams by production of Extended-Spectrum Beta-Lactamases (ESBLs) and to (fluoro)quinolones represent emerging resistance properties which dramatically limit the therapeutic options in both, veterinary and human medicine.Resistant Enterobacteriaceae occur in different animal species, in the environment, in feed and food as well as in humans and are spread between the different reservoirs. Therefore, animals, the environment and food may affect human health. However, the public health impact as well as the transmission mechanisms of resistant Enterobacteriaceae and resistance genes from different origins are not yet fully understood.
This research consortium aims at assessing the impact of different origins, transmission routes and pathogen attributes on the risk for humans being exposed to ESBL-producing and (fluoro)quinolone-resistant Enterobacteriaceae. Thus, most research activities will take place in areas of possible pathogen sources (animals, food) which may have an impact on human health. Existing data and isolates from studies and surveillance activities will be completed and supplemented by epidemiological studies and diagnostic investigations of the RESET consortium. The association between reservoir-related – including humans –, pathogen-related and epidemiological information will contribute to quantify and assess risks which can be integrated in the development of control strategies.
Part of LGL (as associated partner):
Studies along the food chain in Bavaria (WP 2):
Based on results of the project “Monitoring of Antimicrobial Resistance throughout Bavaria�? performed at the LGL, this associated partner will run a cross-sectional study in Bavaria. 60 cattle farms will be selected representatively to cover different production types (cattle fattening farms, dairy farms, veal calf production). In the same region, locally produced animal derived foods (raw meat from cattle, pigs and poultry, raw milk and milk products, raw vegetables) will be tested for presence of E. coli to collect up to 50 isolates per food category for further phenotypic resistance testing. Data collected and samples taken will be in line with other cross sectional studies. Collection and investigation of samples will be performed by LGL and isolates will be typed using agreed harmonised diagnostic, phenotypic and genotypic methods. Isolates will be provided for specific research and data will be provided for overall statistical analyses.
Cross sectional study (WP 2b)
In collaboration with an associated partner (LGL) prevalence of ESBL- E. coli among 5.000 persons of the general population (outpatients) will be assessed by a selective screening of human stool samples. The pre-characterised and ESBL-confirmed isolates will be sent to partner for further characterisation and molecular typing.